This work represents development and validation of novel, accurate, precise, rugged, robust and reproducible high performance thin-layer chromatographic technique for the simultaneous estimation of metoprolol succinate and chlorthalidone from marketed formulation. High performance thin-layer chromatography was performed using HPTLC aluminium plates precoated silica gel plate 60 F254 as stationary phase. The solvent system consisted of Toluene: Ethyl acetate: Methanol: Triethylamine (6: 1: 1: 0.6 v/v/v/v) as the mobile phase for the separation of metoprolol succinate and chlorthalidone. The densitometric analysis of metoprolol succinate and chlorthalidone was carried out at 230 nm. The method was validated as per the International Conference on Harmonization (ICH) guidelines and found to be linear in the range of 500-7000 ng spot−1 for metoprolol succinate and 125-1750 ng spot−1 for chlorthalidone. Rf value for metoprolol succinate and chlorthalidone is 0.53 and 0.24 respectively. The relative standard deviation (% RSD) values of the precision study were <2% which indicated that the developed method was precise; recovery was found to be 99.9% - 100.1% for metoprolol succinate and 99.9% - 100.3% chlorthalidone respectively. The extremely simple mobile phase composition makes this method cost effective, rapid and nontedious. The method can be important tool for simultaneous estimation of both drugs in commercial products for routine analysis and quality control laboratories.
Loading....